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Samples were washed x3 with cold argon-purged 50 mM HEPES buffer (pH 7.5), resuspended in 500 L washing buffer and exposed first to 1 mM TCEP, and after 3 min, 5 mM IAM was added and incubated for a further 20 min (Procedure A) or IAM-TPP (Procedure B) for 30 min at RT protected from light
MCP-1 (CCL2), Monocyte Chemoattractant Protein-1
Distinguishing Direct versus Indirect Transcription Factor-DNA Interactions
10.1093/jxb/ert172 21 DbrowskaG.KataA.GocA.Szechyska-HebdaM.SkrzypekE
10.1126/science.2183352 37 ToppoS.FlohL.UrsiniF.VaninS.MaiorinoM
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