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The mobile phase system was composed of Solvents A (10 mM ammonium acetate, 10 mM ammonium hydroxide in 95% H 2 O/5% ACN) and B (10 mM ammonium acetate, 10 mM ammonium hydroxide in 95% ACN/5% H2O)

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This resulted in reduced WAT mass, increased liver mass, impaired glucose tolerance and elevated circulating FAs, which is consistent with a primary defect in adipose lipid storage