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Subsequently, the cells were washed with PBS and blocked for 1 h with 1% bovine serum albumin (BSA, Sigma A9418) and 22.52 mg/mL glycine in PBST (PBS + 0.05% Tween-20, LPS Solution CBP007T)

The first section was used for a hematoxylin and eosin (HE) staining to score the intensity of infiltration with mononuclear and polymorphonuclear cells

Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving

Id also like to think that Id be able to tell if I was looking overly done

Why this dose+ Reached in phase 2 by a different ladder: 0.6 mg start, doubling every 2 weeks (0.6 1.2 2.4 4.5)