Noyan-Ashraf MH, Shikatani EA, Schuiki I, Mukovozov I, Wu J, Li RK, Volchuk A, Robinson LA, Billia F, Drucker DJ, et al
All cell lines were cultured according to standard protocols and mycoplasma testing was performed monthly using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza, Walkersville, MD, USA)
The calibration was done by incubation of rml1 seedlings expressing GRX1-roGFP2 or GRX1-roGFP2-iL in 10 mM DTT and 25 mM H 2 O 2 , respectively, to drive the roGFPs to their fully reduced and fully oxidized forms
Friedrich N, Schwarz S, Thonack J, John U, Wallaschofski H, Vlzke H
Then Jessica Brown, Dr
The observed improvement arose via two synergistic effects: (1) direct non-covalent interactions of Y218 with the PET substrate, and (2) the subsequent enhancement in enzyme-substrate binding affinity