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The supernatant was isolated by centrifugation at 65,000 g for 30 min and incubated with amylose resin (New England Bio-Labs) for 2 h at 4C

Mortazavi et al

Dams were returned to the cage, and behavior was recorded for 10 min [28]

in pancreatic -cells, high PC2 levels are responsible for the predominant glucagon synthesis (Figure 1) ( Gcg

These brain cells rely on glycolysis in energy generation and produce lactate, which serves as both main energy fuel and a signaling molecule for neurons