When NNMT activity is elevated as documented in obese adipose tissue, several cancer types, and aged skeletal muscle intracellular NAD+ availability decreases, SAM availability decreases, and downstream sirtuin activity (particularly SIRT1 and SIRT3) is reduced
Lean mice did not lose significant weight
So, we will do the best we can with the information we have available
Proper storage and handling help reduce degradation processes such as hydrolysis, oxidation, and peptide aggregation, which can affect experimental reliability
Additional information about the model development is available in the Supplementary Material
Tell your provider if any of these movements hurt and which ones are the most painful