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Cells were plated 5 days after transduction and cultured for 4 days with or without hemin (1 M) and pyruvate (1 mM)

Hereafter, ALP staining and ARS staining were used to verify the effects of Fer-1 on osteogenic function

The results of flow cytometry also demonstrated that, as detected by Annexin staining, FOXO4-DRI treatment significantly promoted apoptosis in senescent cells (Figure 6E)

however, larger, controlled clinical trials are necessary to validate these findings and establish standardized treatment protocols

But this isnt the case

Discussion/Conclusion Although links between redox dysregulation and ALS are becoming well documented in the literature, the directionality of these links and their underlying cause is still unclear