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Briefly, HaCaT keratinocytes were seeded at a density of 10,000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

Prior to administration of the drip, an antiseptic wipe is used to cleanse the injection area in order to reduce the risk of developing any unexpected infection

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Plasma potassium should therefore be monitored during this period

It's important to watch for these signs and talk to a doctor

In contrast, the fraction of the dose excreted in urine increased significantly for both pravastatin and torsemide, indicating that impaired sinusoidal uptake by Slco1b2 is compensated through increased renal excretion in this case (Supplementary Figure S6)