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When comparing more than two groups, one-way ANOVA was applied, followed by Scheffs post-hoc test to identify statistically significant differences

Flowers J, Lonky S, Deitsch E

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DJ-1 inhibits glutathione degradation by downregulating CHAC1 expression in astrocytes

Finally, the pathway analysis was performed using ShinyGO v0.80 83 and Reactome 84 to investigate the enriched pathways associated with the significant proteins identified by the Random Forest method and Volcano plots

Fluorescence/luminescence/absorbance intensities at appropriate wavelengths were quantified in Synergy H1 microplate reader (version 2.09.1)