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Osman AA, Arslan E, Bartels M, Michikawa C, Lindemann A, Tomczak K, et al
The mix was incubated for 2 min and then 1 l of Thermoscript reverse transcriptase (200 U) (Invitrogen) was added and incubated for 50 min at 42 C
Extending it by even a few days, or storing at room temperature "just for convenience," turns a sterile reconstitution protocol into a contamination experiment
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